GTPase-activating properties of ARHGAP11A.
- Yoshinori Kagawa (503599)
- Shinji Matsumoto (503600)
- Yuji Kamioka (503601)
- Koshi Mimori (30872)
- Yoko Naito (201483)
- Taeko Ishii (503602)
- Daisuke Okuzaki (482418)
- Naohiro Nishida (503603)
- Sakae Maeda (503604)
- Atsushi Naito (503605)
- Junichi Kikuta (503606)
- Keizo Nishikawa (503607)
- Junichi Nishimura (206586)
- Naotsugu Haraguchi (503608)
- Ichiro Takemasa (1607)
- Tsunekazu Mizushima (206584)
- Masataka Ikeda (428186)
- Hirofumi Yamamoto (1614)
- Mitsugu Sekimoto (1613)
- Hideshi Ishii (30870)
- Yuichiro Doki (402704)
- Michiyuki Matsuda (108803)
- Akira Kikuchi (202891)
- Masaki Mori (30875)
- Masaru Ishii (257500)
Publication date
December 2013
DOIAbstract
<p>(A) Halo-Tagged ARHGAP11A and Halo-Tag proteins expressed and purified from HEK293 cells. (B) Detection of GTPase activity by quantifying inorganic phosphate released by GTP hydrolysis by Rho family proteins. ARHGAP11A enhanced the GTPase activity of RhoA, but not of Rac1 or Cdc42. (C) Detection of active and total forms of various Rho-family proteins (RhoA, RhoB, RhoC, Rac1, and Cdc42) in HEK293 cells transfected with Halo-ARHGAP11A or its control. Expression of ARHGAP11A reduced the amounts of the active forms of RhoA, RhoB, and RhoC. (D) Assessment of mutant ARHGAP11A without the putative GAP domain (ΔGAP).</p
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