Identification of dysregulated genes after knock-in of miR-199a-5p in NT2 cells.

<p>A). qPCR showing more than four thousand fold changes of miR-199a-5p expression levels after transiently transfection of mimic of miR-199a-5p into NT2 cells comparing to NT2 cells transfected with scrambled control RNA. (Amount of miR-199a-5p mimics or scrambled control transfected: 25 nM). *, p<0.05 miR-199a-5p expression compared miR-199a-5p mimics transfection in NT2 cells with control. B). Western Blot showing reduced expression of PODXL, a confirmed direct target of miR-199a-5p in TGCTs after miR-199a-5p overexpression. C). Heatmap showing hierarchical clustering of differently expressed genes after miR-199a-5p transfection (Fold change>1.2, FDR<0.6).