Functional studies of MAFB in TGCTs.

<p>A). Representative images of immunohistochemistry of MAFB in non-cancerous and malignant testicular tumor sections. B). Testis patient samples were divided into four groups(negative, weak, moderate or strong) based on the MAFB level and the proportion of different grades of tumors showing that MAFB protein is highly expressed in malignant testicular tumor. C) Depletion of MAFB by RNAi suppressed cell proliferation <i>in vitro</i> as revealed by CCK8 assay. *, p<0.05 siMAFB transfected NT2 cells compared with scrambled control transfected cells. D) Scatter plots of miR-199a-5p and miR-199a-3p expression against MAFB level. Expression of miR-199a-5p, but not -3p, correlates negatively with MAFB level (Negative: n = 12; Weak: n = 31; Moderate: n = 24; Strong: n = 26; miR-199a-5p: Spearman correlation = −0.276, <i>p</i> = 0.005; miR-199a-3p: Spearman correlation = −0.027, <i>p</i> = 0.791).