Infection and replication of SINV in HEK293 cells.

<p>(A). Northern blot showing SINV full length genome (49 S) and subgenomic (26 S) positive strand RNA rapidly accumulates in HEK293 cells from 2hpi and increasing through 8 hpi. The probe consisted of P32 end-labelled primers at positions 7568–7631 in the genome. The 28 S and 18 S RNA bands stained with ethidium bromide are displayed to demonstrate equal loading. (B) Immunostaining of SINV in HEK293 cells using rabbit anti E2 glycoprotein antibody detected SINV E2 translation increasing over 4, 6 and 8 hpi with over 95% of cells infected with well-defined replication centres (visualised with an anti-rabbit secondary antibody labelled with Alexa488 and nuclei are shown with DAPI).