GATA-4 and GATA-6 are similarly induced in the adult heart with pressure overload.

<p>A, Western blot analysis of GATA-4 and GATA-6 protein expression in the adult mouse heart at baseline or after pressure overload stimulation for the indicated times. A non-specific (n.s.) band from the GATA-6 western was used to show equal loading and running conditions. B, Relative luciferase units (RLU) from a plasmid containing the luciferase reporter fused to the b-type natriuretic peptide (BNP) promoter or a mutant promoter lacking functional GATA binding sites, which was co-transfected into neonatal rat cardiomyocytes with expression plasmids encoding GATA-4 (G4), GATA-6 (G6), or both together. *P<0.05 versus empty vector transfected control. N = 3. C, Heart weight normalized to body weight (HW/BW) of the indicated genotype of cardiac-specific transgenic mice expressing either GATA4, GATA6, or both together at 8 weeks of age.