Venn diagrams depicting proteome profiles of <i>G. lamblia</i> trophozoites analyzed at 0, 4, 8 and 12hpie.

<p>(A) The proteomic analysis done in triplicates of <i>G. lamblia</i> trophozoites after 4, 8 and 12hpie yielded a total of 960 proteins with at least two unique peptides (further information in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0083207#pone.0083207.s004" target="_blank">Table S2</a>). Although the total number of proteins identified in each of the 4 time-points (in bold) was comparable, mean Spearman rank correlation coefficients (in italics) indicated that the 4hpie time-point differed the most with respect to the other 3 time-points. hpie: hours post induction of encystation. (B) In this study, we identified a total of 1063 proteins, 316 of which were annotated as “hypothetical” in the Giardia Genome Database. Based on its current release (GiardiaDB 3.0; 11^th of March 2013), the <i>G. lamblia</i> assemblage A WB strain genome was assigned 5237 protein-encoding genes, with 3557 genes awaiting annotation.