Mitochondrial shape was not substantially affected in A53T cortical neurons but the length was shortened.

<p><b>A</b>, <b>B</b>, Examples of mitochondrial morphology in wt (<b>A</b>) and A53T (<b>B</b>) cortical neurons at 7DIV. Cortical neurons were transfected with DsRed-mito. Higher magnification images at the bottom were from the areas highlighted by red boxes at the top images. <b>C</b>, Quantification of mitochondrial shape parameters (circularity, roundness and aspect ratio) in wt and A53T neurons at 6-7DIV in one representative experiment. There was no significant change in A53 neurons vs. wt neurons. <b>D</b>, Summary of mitochondrial length and mitochondria width in wt and A53T neurons from one representative experiment. Data plotted as Mean +/- SEM. t-test was used (****p<0.0001). Experiments were repeated 3 times (see results in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085815#pone.0085815.s003" target="_blank">Figure S3</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085815#pone.0085815.s006" target="_blank">tables S1</a> & <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085815#pone.0085815.s007" target="_blank">S2</a>). Scale bar=20μm. wt=wild type, A53T=A53T α-synuclein, μm=micrometers, DIV=days <i>in </i><i>vitro</i>.