Optimizing size-based sorting of HEK293 cells.

<p>(A) The low and high 10% ends of the depicted light-scatter distributions of proliferating HEK293 cells were gated for sorting by FACSAria III. (B) Volume distributions of the sorted low- and high-end cell populations, as determined by Multisizer IV Coulter counter, are plotted in red and black, respectively. We used the calculated percent overlap and the difference in median values (Δ median) between the measured volume distributions of the ‘small’ and ‘large’ sorted cell populations to evaluate the competency of various light-scatter parameters to approximate cell size in HEK293.