RvD1 inhibited phosphorylation of JNK and the Smad3 linker region but not Smad3 c-terminal phosphorylation in the obstructed kidney.

<p>Mice underwent sham or unilateral ureteral obstruction (UUO) surgery. Two days after UUO surgery, mice received intraperitoneal injections of RvD1 for another two days and were killed on day 4. (A) Immunoprecipitation (IP) of Smad3 followed by Western Blotting (WB) identified phosphorylation of the Smad3 C-terminal domain (p-C-Smad3) and linker region (p-T179 and p-S208) following unilateral ureteric obstruction (UUO) compared to the sham operated control. In addition, Smad3 IP pulled down phosphorylated JNK (p-JNK). (B) Western blot identifying phosphorylation of the Smad2 C-terminal domain (p-C-Smad2), total Smad2, total Smad4 and GAPDH. (C) Quantification of the relative levels of abundance of p-JNK, p-T179, p-S208, p-C-Smad3 compared to total Smad3, p-C-Smad2 compared to total Smad2 and total Smad4 compared to GAPDH. Data are mean ±SD from groups of 6 mice and analysis by one-way ANOVA with post hoc analysis with Tukey's multiple comparison test. *** <i>p</i>&lt;0.001; N.S., not significant.