Groups of 6 or 7 Balb/c mice were left unimmunized (PBS) or were immunized intranasally 4 times with <i>H. Pylori</i> lysate+5 µg of CT adjuvant, CTA1-UreB T237–251/B 321–339-DD (a fusion protein containing 1 copy of the T epitope (UreB 237–251)+1 copy of the B epitope (B321–339)), UreB 237–251 alone+5 µg of CT adjuvant, or a mixture of UreB 237–251 and UreB B321–339+5 µg of CT adjuvant.

<p>Four weeks after completion of immunization a separate group of 3 naïve mice (Naïve) were left unchallenged and all other mice were challenged twice over three days with ca 2×10⁷ cfu of <i>H. pylori</i> SS-1 and 7 weeks later mice were killed and recall splenic IFNγ responses in supernatants from lysate-stimulated cultures with unstimulated background controls subtracted (A), serum IgG anti-<i>H. pylori</i> antibody responses (B) and gastric <i>H. pylori</i> cfu/g of stomach tissue (C) were determined. These are representive data from 2 identical experiments giving similar results. ANOVA was used for statistical analysis : *p<0.05, **p<0.01 and ***p<0.001.