Pan-muscular Gemin3 knockdown or Gem3^ΔN overexpression gives rise to identical motor phenotypes.

<p>(<b>A</b>) Compared to controls (<i>Mef2-GAL4>Dcr-2</i> and <i>Gem3-IR^mun/+</i>), the time taken for the first fly to climb 8 cm increases progressively over the course of 35 days in sample populations having reduced levels of Gemin3 in muscles (<i>Mef2-GAL4>Dcr-2+Gem3-IR^mun</i>). The latter genotype also exhibits an age-dependent progressive decline in the total percentage of flies that successfully reached the 8 cm mark within 10 seconds when compared to the same controls. (<b>B</b>) An identical progressive change in climbing behaviour is observed between flies with pan-muscular Gem3^ΔN overexpression (<i>Mef2-GAL4>Gem3</i>^Δ<i>N</i>) and the uncombined driver (<i>Mef2-GAL4/+</i>) or transgene (<i>Gem3</i>^Δ<i>N</i><i>/+</i>) controls. The day 25 and day 35 time points were excluded from the chart due to a lack of difference with the driver control (<i>Mef2-GAL4/+</i>). (<b>C)</b> Flight behaviour was determined using the Droso-Drome, in which the height a fly falls determines its flight performance. Fliers tend to stick to the upper sectors whereas non-fliers drop to sector 1, the lowest sector. On day 5 and day 15 post-eclosion, the percentage of flight-impaired organisms in the Gemin3 knockdown population (<i>Mef2-GAL4>Dcr-2+Gem3-IR^mun</i>) is not significantly different to that of controls. This situation changes on the day 25 and day 35 time points whereby a significant difference is obvious. At day 35, the percentage of flightless flies in the <i>Mef2-GAL4>Dcr-2+Gem3-IR^mun</i> population are in the majority such that significant differences with flies overexpressing Gem3^ΔN (<i>Mef2-GAL4>Gem3</i>^Δ<i>N</i>) do not persist at this time point. Data presented are the mean ± S.E.M. and statistical significance was determined for differences between the <i>Mef2-GAL4>Dcr-2+Gem3-IR^mun</i> (A, C) or <i>Mef2-GAL4>Gem3</i>^Δ<i>N</i> (B) genotype and other genotypes, which are indicated by the respective colour. For all data, ns = not significant, *p<0.05, **p<0.01, and ***p<0.001.