Arsenic and temsirolimus combination treatment results in decreased phospho-S6 and ERK activation.

<p>MDA-MB-468, MCF-7, SkBr3, and T47D cell lines were exposed to vehicle control (Vh; 5.3 x 10^-6 N NaOH and 2.5 x 10^-5 % EtOH in PBS), 1-2 µM ATO, 0.5-5 ng/ml temsirolimus and the combinations for 24 hours. Whole cell extracts were used in immunoblotting experiments for phospho-S6, S6, phospho-ERK, ERK, and β-actin. Immunoblots shown are representative of experiments performed at least 3 times.