BMSCs inhibit cytokine and chemokine production by activated microglia.

<p>We analyzed cytokine and chemokine production by microglia using Bio-Plex Pro assays. (A) LPS-treated microglia produce more TNF-α, IL-1β, RANTES and MIP-2 than control microglia, while treatment with conditioned medium significantly inhibited production of these factors. (B) Microglia in the LPS-treated groups produced more IL-6, IL-10 and MCP-1 compared to control groups. The supernatant derived from conditioned medium-treated groups contained more IL-6, IL-10, MCP-1 and VEGF compared to control groups. A P value of less than 0.05 (*), less than 0.01 (**), and less than 0.001 (***) was considered statistically significant.