AR activation alters the miRNA profile of PC3-AR cells.

<p>(A) Western blot to confirm inducible AR expression in PC3-AR cells. PC3-AR cells were treated 5 days with doxycycline to induce AR expression and with R1881 to induce AR activation and nuclear translocation. The comparison is to untreated control. Total cell lysates were used for analysis. (B). Heat map of miRNA expression in PC3-AR and control cells. Total RNA from cells in A was used for microarray analysis and each sample analyzed in triplicate. The statistical significance for expression changes shown has been determined using Student’s T-test. P values <0.05 were attributed statistical significance.