Detection of antibody secreting cells (ASCs).
- Kar Muthumani (504713)
- Megan C. Wise (504714)
- Kate E. Broderick (396225)
- Natalie Hutnick (252194)
- Jonathan Goodman (504715)
- Seleeke Flingai (504716)
- Jian Yan (49839)
- Chaoran B. Bian (504717)
- Janess Mendoza (504718)
- Colleen Tingey (504719)
- Christine Wilson (504720)
- Krzysztof Wojtak (504721)
- Niranjan Y. Sardesai (49844)
- David B. Weiner (49846)
Publication date
December 2013
DOIAbstract
<p>Groups of mice (<i>n = 4</i>) were immunized with the multi-clade constructs. (A) 96-well plates were coated with goat anti-mouse IgG in PBS and blocked overnight at 4°C. Approximate number of IgG producing B-cells was determined by ELISpot assay. (B) Representative plots of two individual experiments are shown; error bars represent standard deviation of at least three replicate wells. (C) Correlation between the binding antibody titers and SFU obtained by B-cell ELISpot assay.</p
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