<p>The <i>T<sub>m</sub></i> values of MIPSs were determined using DSF. The <i>T<sub>opt</sub></i> values were determined using a MIPS/malachite green assay. Standard deviations for MIPS <i>T<sub>m</sub></i> values were determined from three replicates. <i>T<sub>opt</sub></i> values were determined from at least four replicates. ND, not determined.</p
W for wild type lipase; M1 for mut1 and M2 for mut 2 lipases. The mid-point of fluorescence transiti...
Increase in fluorescence was detected as hydrophobic regions of the protein were exposed as the prot...
<p>(a,b,c) Different strategies of thermal adaptation of hypothetic proteins. (d) Comparison between...
<p>The thermostability of each virus was determined by incubation at 45°C for various times and quan...
<p>Basal and acquired thermotolerance values of seven durum wheat varieties estimated by cell membra...
Abstract Background The ability to design thermostable proteins is theoretically important and pract...
<p>Activities of enzymes were calculated using Lineweaver-Burk plots. MIPS assays were done at each ...
<p>Starting with the shared ancestor, Anc1, stabilities increase along the thermophilic lineage and ...
<p>MZ 5<sub>B</sub> from Group A and E<sub>9 m</sub> from Group B were independently replicated 5 an...
<p>A) Protein samples were incubated at different temperatures for 30 minutes and the remaining fluo...
<p>(<b>a</b>) Thermostability of unpurified digitonin-solubilized, <sup>125</sup>I-AngII-bound AT<su...
<p>Summary of the biophysical parameters of <i>Pf</i>MDH mutants, as assessed by Static Light Scatte...
Engineering proteins to enhance thermal stability is a widely utilized approach for creating industr...
<p><b>(A)</b> The residual activities of the enzyme were measured after incubation at 45°C for diffe...
<p>(a) A scatter plot representing a subset of mutations (45 out of the total 200). The plot was gen...
W for wild type lipase; M1 for mut1 and M2 for mut 2 lipases. The mid-point of fluorescence transiti...
Increase in fluorescence was detected as hydrophobic regions of the protein were exposed as the prot...
<p>(a,b,c) Different strategies of thermal adaptation of hypothetic proteins. (d) Comparison between...
<p>The thermostability of each virus was determined by incubation at 45°C for various times and quan...
<p>Basal and acquired thermotolerance values of seven durum wheat varieties estimated by cell membra...
Abstract Background The ability to design thermostable proteins is theoretically important and pract...
<p>Activities of enzymes were calculated using Lineweaver-Burk plots. MIPS assays were done at each ...
<p>Starting with the shared ancestor, Anc1, stabilities increase along the thermophilic lineage and ...
<p>MZ 5<sub>B</sub> from Group A and E<sub>9 m</sub> from Group B were independently replicated 5 an...
<p>A) Protein samples were incubated at different temperatures for 30 minutes and the remaining fluo...
<p>(<b>a</b>) Thermostability of unpurified digitonin-solubilized, <sup>125</sup>I-AngII-bound AT<su...
<p>Summary of the biophysical parameters of <i>Pf</i>MDH mutants, as assessed by Static Light Scatte...
Engineering proteins to enhance thermal stability is a widely utilized approach for creating industr...
<p><b>(A)</b> The residual activities of the enzyme were measured after incubation at 45°C for diffe...
<p>(a) A scatter plot representing a subset of mutations (45 out of the total 200). The plot was gen...
W for wild type lipase; M1 for mut1 and M2 for mut 2 lipases. The mid-point of fluorescence transiti...
Increase in fluorescence was detected as hydrophobic regions of the protein were exposed as the prot...
<p>(a,b,c) Different strategies of thermal adaptation of hypothetic proteins. (d) Comparison between...