Mutational effects on global gene expression.

<p>A litter of 14.5-dpc fetuses were prepared from a crossing of a heterozygote male (−^m/+) and wild-type female. Global gene expression analyses were performed with a set of total 8 embryo heads and placenta representing one of the following four combinations: male hetetrozygote and wild-type and female heteterozygote and wild-type. This series of expression analyses identified four groups of genes: up- and down-regulated gene sets for embryo head and placenta. These four groups of genes were used for identifying enriched pathways using the bioinformatics program EGAN (Explorative Gene Association Networks). Two of these four results are shown: enriched pathways with up-regulated genes at 14.5-dpc embryo heads (<b>A</b>) and placenta (<b>B</b>). The remaining two results showing enriched pathways with down-regulated genes at both samples are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0083359#pone.0083359.s001" target="_blank">Fig. S1</a>. The annotation for the diagrams is as follow. Lines: Dark Purple, Human protein-protein interactions; Light Purple, PubMed co-occurrence (literature); Gold, All protein-protein interactions; Grey, Chromosomal sequence (proximity); Red, GO Function, transcription regulator activity; Green, GO Process, generation of neurons. Circles: Red (color gradient), up-regulated genes; darker red signifying higher WT∶KO expression ratio.