Effects of DKK1 knockdown in LTEP-a-2 cell.

<p>(A) RT-PCR and Western blot analysis of the DKK1 expression. DKK1 level in LTEP-a-2 cell transfected with pSilencer-DKK1 is significantly lower than that in pSilencer-NC and blank control group. (B) The wounded and healing LTEP-a-2 cells. (C) Measurement of migration distance (*<i>P</i><0.05). (D) The invasion ability of LTEP-a-2 transfected with pSilencer-DKK1 was significantly repressed. (E) The number of cells migrating through the Matrigel-coated filters was statistic analyzed. Assays were done in triplicate wells (*<i>P</i><0.01).