BK stimulates translocation/phosphorylation of NF-κB in circulating proangiogenic cells.

<p>(A) p65 (NF-κB) phosphorylation following exposure to BK (1 µM, 15 min) in presence/absence of fasitibant (0.1 µM). Gel are representative of three experiments. The ratio between p-p65 over p65 is reported. *p<0.05 compared to untreated cells; ###P<0.001 to BK-treated cells. (B) Immunofluorescence (40 X) of NF-κB translocation in PACs in 0.1% FBS (a), BK (1 µM) (b), fasitibant (0.1 µM,) (c), fasitibant+BK (d). Bar = 100 µM. (C) COX-2 expression in human hematopoietic progenitor cells pretreated for 30 min with IKK inhibitor VII (0.2 µM) or with fasitibant (0.1 µM), and treated with BK (1 µM, 6 hrs). Gel is representative of three experiments. The ratio between COX-2 over actin is reported. ***p<0.001 compared to untreated cells; ###P<0.001 to BK-treated cells. (D) PGE-2 release from PACs treated with BK (1 µM) in presence/absence of fasitibant (0.1 µM) or IKK inhibitor VII (0.2 µM), for 8 hrs. Numbers represent mean ± SEM of three experiments. *p<0.01, compared to untreated cells; #P<0.01 to BK-treated cells.