Quantifying the penetrance and expressivity of CRM activity.

<p>(A) Automated image analysis workflow. The gene expression pattern was digitized to create a mask for the tissue of interest. Comparing the mask with CRM activity enabled rapid and reliable scoring of both penetrance and expressivity. Errors in penetrance and expressivity were estimated as described in Supplemental Methods (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004060#pgen.1004060.s016" target="_blank">Text S1</a>). Penetrance (red bars) and expressivity (blue bars) of pMad-Tin heterotypic CRMs in the VM (B) and the heart (C). Note that <i>P</i>-element pMad-Tin A2 line was used to quantify heart activity.