<p>Primers used for the cloning of nine Pks domains in plasmid pV1J.ns-tPA. Restriction enzymes used are indicated. Sense primers are listed first and antisense primers second, within primer pairs. All restriction sites are shown in bold.</p
<p>Underlined regions represent restriction enzyme sequences</p><p>Primers and probes used in this s...
<p>Note: Kan<sup>r</sup> and Spc<sup>r</sup>, for kanamycin and spectinomycin resistance, respective...
<p>a. Introduced restriction sites are underlined. Amino acid spacer (GGGS) at the N-terminus of GFP...
<p>Restriction enzyme sites are underlined.</p><p>Additional nucleotides added to facilitate restric...
a<p>Underlined letters indicate the restriction enzyme recognition sites, used for cloning purposes....
*<p>Restriction enzyme cleavage sites are marked in bold. The DNA uptake sequence is underlined.</p
<p>These plasmids were verified by DNA sequencing. Underlined nucleotides indicate the designated si...
<p>The underlined sequence contains the restriction enzyme sites added for cloning purposes. Primers...
<p>Note: <sup>1</sup>The sequences in bold print are the integrated restriction enzyme sites.</p
Primers used in this study(underlined in the primer sequences were restriction enzyme sites).</p
1<p>Underlined letters highlight engineered restriction enzyme (RE) sites (names of the introduced R...
<p>Restriction sites are underlined.</p><p>List of forward and reverse primers used in cloning.</p
<p>Note:</p>a<p>The restriction enzyme site XbaI locates downstream of this primer.</p>b<p>The seque...
<p>*The restriction sites in the sequences are indicated by lower cases. The corresponding restricti...
<p>The sequences of primers used in this study, the restriction enzyme sites used in the study are u...
<p>Underlined regions represent restriction enzyme sequences</p><p>Primers and probes used in this s...
<p>Note: Kan<sup>r</sup> and Spc<sup>r</sup>, for kanamycin and spectinomycin resistance, respective...
<p>a. Introduced restriction sites are underlined. Amino acid spacer (GGGS) at the N-terminus of GFP...
<p>Restriction enzyme sites are underlined.</p><p>Additional nucleotides added to facilitate restric...
a<p>Underlined letters indicate the restriction enzyme recognition sites, used for cloning purposes....
*<p>Restriction enzyme cleavage sites are marked in bold. The DNA uptake sequence is underlined.</p
<p>These plasmids were verified by DNA sequencing. Underlined nucleotides indicate the designated si...
<p>The underlined sequence contains the restriction enzyme sites added for cloning purposes. Primers...
<p>Note: <sup>1</sup>The sequences in bold print are the integrated restriction enzyme sites.</p
Primers used in this study(underlined in the primer sequences were restriction enzyme sites).</p
1<p>Underlined letters highlight engineered restriction enzyme (RE) sites (names of the introduced R...
<p>Restriction sites are underlined.</p><p>List of forward and reverse primers used in cloning.</p
<p>Note:</p>a<p>The restriction enzyme site XbaI locates downstream of this primer.</p>b<p>The seque...
<p>*The restriction sites in the sequences are indicated by lower cases. The corresponding restricti...
<p>The sequences of primers used in this study, the restriction enzyme sites used in the study are u...
<p>Underlined regions represent restriction enzyme sequences</p><p>Primers and probes used in this s...
<p>Note: Kan<sup>r</sup> and Spc<sup>r</sup>, for kanamycin and spectinomycin resistance, respective...
<p>a. Introduced restriction sites are underlined. Amino acid spacer (GGGS) at the N-terminus of GFP...