Genetic linkage by QTL and concentrations of metabolites^‡ (µM ± s.d.) observed in parasite extracts from chloroquine sensitive (CQS) and resistant (CQR) transgenic parasites.

<p>Peptides are listed by their standard abbreviations, unknowns are abbreviated as Ukn, and 6PGn indicates 6-phosphogluconate.</p><p>Threshold LOD scores for genome wide significance, computed by permutation, before and after Bonferroni α correction for multiple comparison testing. Q-value analysis of the original unfiltered mass list established a LOD score of 5.5 as the 5% false discovery threshold.</p><p><i>p</i>-values for QTL analysis reflect the genome-wide significance computed by permutation (N = 1000) of the sample genotypes (N = 36); <i>p</i>-values reported for metabolite concentrations observed in the parasite extracts were computed by one-way analysis of variance (N = 9; K = 3).</p><p>Metabolite levels are reported as µM concentrations present in 1∶4 final dilutions of extracts from packed infected cells (∼90% parasitemia) as measured by single reaction monitoring mass spectrometry. C2 parasites carry the CQS HB3 <i>pfcrt</i> allele, C4 and C6 carry the CQR <i>pfcrt</i> alleles from Dd2 and 7G8, respectively.