<p>C57BL6/J mice were infected intraperitoneally with 1000 PFU of the indicated viruses and splenocytes were analyzed on day 14 post infection. (A) Representative flow plots illustrating the gating strategy. Splenocytes were gated to remove doublets following gating on the live cell population using a live/dead stain (<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003858#s4" target="_blank">Materials and Methods</a>). (B) Frequency of live, singlet YFP+ cells that were IRF4 positive between M2stop.HY and M2MR.HY viruses. Data shown is from individual mice with five mice per virus group.</p
<p>(A and B) Insertion of a YFP expression cassette into the viral genome, or addition of an AU1 epi...
<p>C57BL/6 mice were infected at 1000 PFU by the intranasal route with the indicated viruses. <b>(A)...
<p>Mice were infected intranasally with 1,000 pfu of MHV68-H2bYFP and splenocytes were harvested at ...
<p>Mice were infected intranasally with either 1,000(low dose) or 4×10<sup>5</sup> pfu (high dose) o...
<p>C57Bl/6 mice were intranasally infected with 5×10<sup>5</sup> pfu of the indicated virus and sple...
<p>A) Limiting dilution PCR analysis to determine the frequency of viral genome positive splenocytes...
<p>(A–D) C57BL6 mice were infected with 1000PFU of the indicated virus either intranasally (A and C)...
<p>YFP+ and YFP− splenocytes from mice were sorted at 42 days post-infection from mice infected with...
<p>C57BL/6 mice were infected at 1000 PFU by the intraperitoneal route with the indicated viruses. <...
<p>Mice were infected at 1000PFU IP with the parental MHV68 H2bYFP virus and two independent clones ...
<p>(A–D) C57BL6 mice were infected with 1000PFU of the indicated virus either intranasally (A–B) or ...
<p>C57Bl/6 mice were infected with 5×10<sup>5</sup> pfu/IN of the indicated virus and spleens were h...
<p>(A) As presented in the FACS plot, infected (CD19<sup>+</sup> YFP<sup>+</sup>, black bars) and no...
<p>(A-B) CD69 and CD25 expression by SG CD4 T cells was assessed 10 days pi by flow cytometry and da...
<p>Mice were intranasally infected with 1,000 pfu of MHV68-H2bYFP and splenocytes were harvested at ...
<p>(A and B) Insertion of a YFP expression cassette into the viral genome, or addition of an AU1 epi...
<p>C57BL/6 mice were infected at 1000 PFU by the intranasal route with the indicated viruses. <b>(A)...
<p>Mice were infected intranasally with 1,000 pfu of MHV68-H2bYFP and splenocytes were harvested at ...
<p>Mice were infected intranasally with either 1,000(low dose) or 4×10<sup>5</sup> pfu (high dose) o...
<p>C57Bl/6 mice were intranasally infected with 5×10<sup>5</sup> pfu of the indicated virus and sple...
<p>A) Limiting dilution PCR analysis to determine the frequency of viral genome positive splenocytes...
<p>(A–D) C57BL6 mice were infected with 1000PFU of the indicated virus either intranasally (A and C)...
<p>YFP+ and YFP− splenocytes from mice were sorted at 42 days post-infection from mice infected with...
<p>C57BL/6 mice were infected at 1000 PFU by the intraperitoneal route with the indicated viruses. <...
<p>Mice were infected at 1000PFU IP with the parental MHV68 H2bYFP virus and two independent clones ...
<p>(A–D) C57BL6 mice were infected with 1000PFU of the indicated virus either intranasally (A–B) or ...
<p>C57Bl/6 mice were infected with 5×10<sup>5</sup> pfu/IN of the indicated virus and spleens were h...
<p>(A) As presented in the FACS plot, infected (CD19<sup>+</sup> YFP<sup>+</sup>, black bars) and no...
<p>(A-B) CD69 and CD25 expression by SG CD4 T cells was assessed 10 days pi by flow cytometry and da...
<p>Mice were intranasally infected with 1,000 pfu of MHV68-H2bYFP and splenocytes were harvested at ...
<p>(A and B) Insertion of a YFP expression cassette into the viral genome, or addition of an AU1 epi...
<p>C57BL/6 mice were infected at 1000 PFU by the intranasal route with the indicated viruses. <b>(A)...
<p>Mice were infected intranasally with 1,000 pfu of MHV68-H2bYFP and splenocytes were harvested at ...