Expression of <i>GRM1</i> and mGluR1 in the MCF10 progression series.

<p><b>A. <i>GRM1</i> mRNA expression.</b> RNA lysates collected from the progression series cells were synthesized to cDNA and qRT-PCR analysis performed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081126#s2" target="_blank">Materials and Methods</a>. Relative fold change <i>GRM1</i> expression of MCF10AT1, MCF10DCIS.com, and MCF10CA1d are compared to MCF10A cells. <b>B. mGluR1 protein expression.</b> Protein lysates were immunoprecipitated with mGluR1 antibody as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081126#s2" target="_blank">Materials and Methods</a>. Premalignant MCF10AT1 and malignant MCF10DCIS.com, and MCF10CA1d express increased levels of <i>GRM1</i> and mGluR1 compared to parental MCF10A cells, with MCF10DCIS.com expressing the highest levels.