Systematic cell segmentation errors caused by increased fluorescence intensity in some dying cells.

<p>This data is generated from images of one cell measured having substantial growth but which eventually died rather than divide. Plotting image intensity along cell cross-sections allows more precise localization of cell boundaries. While in the transmission images growth cannot be substantiated, it is interesting that the fluorescence image shows significant increase in intensity and fluorescing area, that appears to extend beyond the boundary of the cell. The red contours were generated by TrackAssist based on difference to normalized background intensity. The dilation of the segmented area appears to be a genuine feature of the cell, but no longer representative of cell area.