Ang-1 and Ang-2 enhance the TNF-induced expression of chemokines and cytokines.

<p>(A) mRNA expression profiles angiogenesis related genes in macrophages differentiated with GM-CSF, IFN-γ or IL-10 after 4 h incubation in medium alone or TNF-α (10 ng/ml) in the absence or presence of Ang-1 (200 ng/ml) or Ang-2 (200 ng/ml) (n = 3). Data is presented as a heat map where lowest mRNA expression is showed in dark blue and highest in yellow. (B) Analyses of chemokines and cytokines mRNA expression levels of selected genes analyzed in (A). Data is shown as log₂ relative quantity respect to unstimulated cells, as described in material and methods. Bars represent the means and SEM of 3 independent experiments. *P<0.05, between stimulatory conditions. Friedman test was used for statistical analyses. (C) Multiplex analysis of protein production by macrophages differentiated in GM-CSF, IFN-γ, or IL-10 after 24 h incubation in medium alone or TNF (10 ng/ml) in the absence or presence of Ang-1 (200 ng/ml) or Ang-2 (200 ng/ml). Bars represent the means and SEM of 6 independent experiments. *P<0.05, * P<0.01, between stimulatory conditions, #P<0.05, ##P<0.01, ### P<0.001, compared to unstimulated cells. Friedman test was used for statistical analyses.