Ang-1 and Ang-2 fail to induce macrophage polarization.

<p>(A–D) FACS analysis of expression of macrophage surface markers (A) CD16, (B) CD163, (C) CD200R and (D) CD64 in macrophages differentiated for 7 d in medium alone, or medium containing the indicated polarizing cytokines, Ang-1 (200 ng/ml) or Ang-2 (200 ng/ml). Data in A–D are presented as the geomean normalized to values obtained for macrophages cultured in medium alone, and represent the mean ± SEM of 4–5 independent experiments per marker. (E) Surface protein expression (geomean) and mRNA expression (F) of Tie2, as determined by FACS analysis (n = 5) and qPCR (n = 3), respectively, in human macrophages cultured for 7 d in the absence (medium) or presence of 200 ng/ml Ang-1 or Ang-2. *P<0.05, **P<0.01, ***P<0.001, versus macrophages differentiated in medium alone. Kruskal-Wallis test was used for statistical analyses.