Distinct expression pattern of CBLN4 during embryonic development and subtle effects on axon pathfinding in <i>CBLN4−/−</i> mice.

<p>A) Lac-Z reporter expression of CBLN4 in E11.5 <i>CBLN4^+/−</i> mice (top panels) showing distinctive expression in limbs including dorsal limb expression. Lac-Z reporter staining of CBLN4 in E13.5 <i>CBLN4^+/−</i> mice (lower panels) showing expression in the spinal cord motor column. B) commissural axons of E11.5 spinal cord visualized with TAG-1 immunostaining in combinations of <i>CBLN4</i> and <i>Netrin-1</i> genotypes. C) Representative images and quantification of wandering axons (white arrows) in the brachial plexus of E11.5 <i>CBLN4^−/−</i> mice. Tukey contrasts; **, <i>P</i><0.01; ***, <i>P</i><0.001; n is left and right forelimb nested in mouse; n = 4 <i>CBLN4^+/+</i>; n = 5 <i>CBLN4^+/−</i>; n = 6 <i>CBLN4^−/−</i>.