CBLN4 binds specifically with DCC on cells.

<p>A) Representative images of CBLN4-AP protein binding to COS-7 cells transfected with DCC, but not NgR expression constructs (top panels). Nogo 66-AP protein bound to NgR but not to DCC transfected cells (middle panels). AP did not interact with either DCC or NgR (lower panels). B) Flow cytometric analysis confirmed cell-surface expression of transiently transfected Flag-tagged DCC on HEK239T cells (top panels). The CBLN4-Fc and gD-Netrin-1 proteins bound to DCC expressing cells as demonstrated by the shift in fluorescent intensity compared to untransfected cells (bottom panels).