ROS content and expression pattern of miR398 and target genes <i>CSD1</i> and <i>Nod19</i> in roots inoculated with <i>Rhizobium tropici</i>.

<p>Measurements were done at initial time (0 h) and 3, 6, 12, 24 and 48 h after inoculation with <i>R. tropici.</i> (A) Histological (fluorescence) detection of ROS accumulation in inoculated root tips using 2′,7′- dichlorodihydrofluorescein diacetate (H₂DCF-DA). The values in parenthesis indicate the average integrated fluorescence intensity per unit area of root tissue ±SD. Asterisk: Student's <i>t</i> test, <i>P</i>≤0.05. Relative expression, determined by qRT-PCR, of (B) miR398b (blue) and of (C) target genes <i>CSD1</i> (green) and <i>Nod1</i>9 (red) in inoculated roots at the indicated time points. Values were normalized to the value from the C condition that was set to 1 as indicated with a dashed line. Values represent the average ± SD from three biological replicates.