Progression of pathology in the midgut of <i>dsDvSnf7</i>-treated WCR larvae.

<p>Observed symptoms were summarized in four phases (1 = lightest to 4 = most severe). Highlighted are macroautophagic complexes (blue ellipses) (diagram, A′), gut lumen (L with orange glowing background) filled with cellular debris and loss of microvilli (white arrowheads) (diagrams, B, B″), large vacuoles (diagrams, B′, B″, C′) (green arrows), luminal membrane disintegration (black arrowheads) (diagram, C″) and infolding of the basal membrane (transparent black arrowheads) (diagrams, C″, D′, D″), progressive cell sloughing (asterisks) (diagrams, A″, B, C). Panels E-E″ show midgut of dsGFP-treated control larvae. Highlighted are endosomes (white arrows), lysosomes (magenta arrows) and autolysosomes (black arrow). refers to grey area denoting sectional artifact (cracks in the tissue section). Scale bars = 4 µm (A-E) and 2 µm (A′-E′ and A″-E″).