Secretions of End1/E6E7 cells modulate cytokine levels by MDMs in response to LPS.

<p>Monocyte derived macrophages (MDMs) were incubated for 24 hrs with fresh medium (No-SM) or spent media from untreated (UT-SM), CpG-ODN (CpG-SM) or poly (I: C) LL (poly-SM) treated End1/E6E7 cells. Later MDMs were stimulated with LPS (100 ng/ml) for an additional 24 hrs and supernatants were analyzed for cytokines viz., TNF-α (<b>A</b>), IL-10 (<b>B</b>), GM-CSF (<b>C</b>), IFN-γ (<b>D</b>) and IL-2 (<b>E</b>) levels Values represent mean ± SD of three experiments performed on different days. Level of significance (*p<0.05; **p<0.01; ***p<0.001; n.s: not significant) was calculated by ANOVA test followed by Bonferroni analysis.