Epithelial cell derived TGF-β mediates anti-inflammatory effects on MDMs.

<p>(A) Spent media (UT-SM) was incubated with anti-TGF-β antibody at varying concentrations for 1 hr at 37°C and was then incubated with MDMs for 24 hrs. Post incubation, MDMs was stimulated with LPS for 24 hrs and secretion of TNF-α was quantitated by ELISA. (B) MDMs were stimulated with recombinant human TGF-β (0.5–2 µg/ml) for 24 hrs and further stimulated with LPS for 24 hrs. Secretion of TNF-α was quantitated by ELISA. Treatment with recombinant TGF-β decreased secretion of TNF-α from MDMs in a dose dependent manner. (C) Spent media of End1/E6E7cells stimulated with CpG-ODN or Poly-(I:C)LL were analyzed for secretion of TGF-β after 24 hrs of stimulation. Values represent mean ± SD of three experiments performed on different days Level. Of significance (*p<0.05; **p<0.01; *** p<0.001 was calculated by ANOVA test followed by Bonferroni analysis.