Bioluminescent imaging of of Rag2^−/− γc^−/− mice intrafemorally transplanted with low numbers of transduced TC-71 cells.

<p>Weekly bioluminescent imaging of 3 Rag2^−/− γc^−/− mice transplanted with 1×10³ (top row), 5×10³ (middle row) or 1×10⁴ (bottom row) transduced TC-71 cells (EGFP sorted 1 week prior to injection). Injected cells were suspended in a volume of 10 µl. The images obtained on day 7 did show a low signal over the right femur for all of the mice, but due to the chosen radiance settings to enable comparison with subsequent iamges, they do not appear on this panel. On some images of mice who were not imaged individually towards the end of the experiment (i.e. middle row day 27 and 41), the reflection of a signal emitted from a neighbouring mouse is unfortunately projected onto the left femur (day 27) or left side (day 41) of that mouse. All mice developed distant disease, either in lungs (top row), liver/abdomen (middle and bottom row) or contralateral leg (middle row).