Anti-MUC1 SP antibodies bind MUC1-expressing MM cells in fresh human BM aspirates.

<p>The cell surface presence of the MUC1 SP domain was evaluated by gated FACS analysis on PC cells in fresh BM aspirates obtained from MM patients (A–E) and normal naïve sample (F–H). Polyclonal anti-MUC1 SP (R23IgG) was used to determine MUC1 SP expression; MUC1 TRA domain expression was determined with H23 mAb. For each experiment, species-matched control antibodies for MUC1 staining were used: either normal mouse IgG–FITC (B), or normal rabbit IgG-FITC (D). Anti-MUC1 SP mAb (SPmAb-2.1) was used to determined MUC1 SP expression; MUC1 TRA domain expression was determined with H23 mAb.