Effect of niclosamide treatment on primary tumor growth and pulmonary metastasis.

<p>(A) 4T1 tumor-bearing female BALB/c mice were treated as described with vehicle, niclosamide at 10 and 20 mg/kg, the mean tumor volumes ± SD of six mice per every group. (B) After 28 days of tumor cell inoculation, the body weight of the niclosamide treatment and vehicle groups were statisticed, and there were no significant difference among the groups. (C) Lung metastatic nodules were visualized to show the inhibitory effect of niclosamide on 4T1 tumor 21 days after treatment. Arrow indicated metastatic nodules (up), The H&E staining of lungs from each group (10×). (D) The mean lung metastasis nodules of each group, the treatment with niclosamide at 20 mg/kg resulted in significant inhibition of lung metastasis versus vehicle control. Bars showed means ± SD (n = 6; *P<0.05).