Niclosamid induces 4T1 breast cancer cells apoptosis.

<p>(A) 4T1 cells were treated with niclosamide at indicated doses for 24 hours, and the level of apoptosis was evaluated using the Annexin V/PI dual-labeling technique, as determined by FCM. Data shown are representative of three independent experiments. (B) Statistic results of apoptosis assays, 4T1 cells positive for both Annexin V and PI were considered apoptotic. Data are expressed as means ± SD from three independent experiments (*p<0.05; **p<0.01). (C) Western blot analyses of 4T1 cells treated (24 h) with different concentrations of niclosamide to evaluate protein expression of Bcl-2, Mcl-1, Cleaved caspase-3, Survivin and β-actin was employed as a standard.