<p>Each week (up to 4 weeks of culture) pancreatic islets cultured alone or directly co-cultured with MSCs, and MSCs cultured alone were exposed to different glucose concentrations in the culture medium (20 mM and 1,67 mM Glucose), and the insulin release after each change was measured by an ELISA assay specific for this hormone. Results are expressed as mean ± SD of three independent experiments. * <i>P</i><0.05 Islets vs Islets+MSCs, <sup>○○ </sup><i>P</i><0.01 Islets+MSCs vs MSCs.</p
<p>A: Morphological changes of the islets cultured with or without MSC at 4 d after starting the cul...
<p>Islets were cultured in cCMRL, 5 or 8 mM glucose with or without the concentrations of MSDC-0160±...
Rat islets were maintained for 3 days in tissue culture at 2.8, 8.3, or 16.7 mM glucose, in the pres...
<p>Glucose-stimulated insulin secretion was tested at three subsequent concentrations of glucose (3 ...
<p>Isolated murine islets were cultured for 24 h at 7 mmol/l glucose (7G) in the absence or presence...
<p>Functional glucose-stimulated insulin release from rat pancreatic islets cultured in standard 2D ...
<p>Insulin release (ng/10 islets*hour) from adhered islets after 2 days <b>(A)</b> and 5 days <b>(B)...
Pancreatic islet isolation and culture technique are tools for direct investigation of the effects o...
<p>SI is the insulin stimulation index i.e. incremental folds above baseline insulin release. Result...
<p>Effect of GLP-1 on the kinetics of a subsequent glucose-stimulated insulin release from rat islet...
BackgroundIn vitro experiments using only β-cell lines instead of islets are limited because pancrea...
Observing different kinetics of nutrient-induced insulin secretion in fresh and cultured islets unde...
<p>Islets were isolated from neurolgin-2 knockout (KO) and wild-type (WT) mice and allowed to recove...
Islets of Langerhans were isolated by collagenase digestion from the pancreas of a 39 year-old femal...
The aim of the present study was to clarify whether prolonged in vitro exposure of human pancreatic ...
<p>A: Morphological changes of the islets cultured with or without MSC at 4 d after starting the cul...
<p>Islets were cultured in cCMRL, 5 or 8 mM glucose with or without the concentrations of MSDC-0160±...
Rat islets were maintained for 3 days in tissue culture at 2.8, 8.3, or 16.7 mM glucose, in the pres...
<p>Glucose-stimulated insulin secretion was tested at three subsequent concentrations of glucose (3 ...
<p>Isolated murine islets were cultured for 24 h at 7 mmol/l glucose (7G) in the absence or presence...
<p>Functional glucose-stimulated insulin release from rat pancreatic islets cultured in standard 2D ...
<p>Insulin release (ng/10 islets*hour) from adhered islets after 2 days <b>(A)</b> and 5 days <b>(B)...
Pancreatic islet isolation and culture technique are tools for direct investigation of the effects o...
<p>SI is the insulin stimulation index i.e. incremental folds above baseline insulin release. Result...
<p>Effect of GLP-1 on the kinetics of a subsequent glucose-stimulated insulin release from rat islet...
BackgroundIn vitro experiments using only β-cell lines instead of islets are limited because pancrea...
Observing different kinetics of nutrient-induced insulin secretion in fresh and cultured islets unde...
<p>Islets were isolated from neurolgin-2 knockout (KO) and wild-type (WT) mice and allowed to recove...
Islets of Langerhans were isolated by collagenase digestion from the pancreas of a 39 year-old femal...
The aim of the present study was to clarify whether prolonged in vitro exposure of human pancreatic ...
<p>A: Morphological changes of the islets cultured with or without MSC at 4 d after starting the cul...
<p>Islets were cultured in cCMRL, 5 or 8 mM glucose with or without the concentrations of MSDC-0160±...
Rat islets were maintained for 3 days in tissue culture at 2.8, 8.3, or 16.7 mM glucose, in the pres...