Amino acid compositions in peptidoglycans and lysozyme MICs of J1 phage-resistant mutants.

<p>^a Amino acid compositions in peptidoglycans were analyzed by high-performance liquid chromatography after samples had been hydrolyzed for 16 h with 6 M HCl at 100°C. Assays were performed in two independent experiments, and the differences of each molar ratio between experiments were less than 10%.</p><p>^b The cells of <i>L. casei</i> ATCC 27139 and isogenic Tn<i>5</i> insertion mutants (1.0×10⁷ CFU/ml) were inoculated into fresh ILS broth containing several concentrations of lysozyme. After incubation at 37°C for 48 h, the minimal inhibitory concentration of lysozyme at which <i>L. casei</i> strains failed to grow was regarded as its MIC.