Plasma pharmacokinetics and brain uptake of 2B3-101 and pegylated liposomal doxorubicin in healthy mice.

<p>(A) Plasma concentration after 1 IV dose (5 mg/kg in 3 mice per group per time point) decreases with time for both liposomal formulations. After 4 days, less than 1% of the amount measured in plasma at 4 h after administration was present in the circulation. (B) The brain concentration decreases in time in line with the plasma level. (C) The brain∶plasma ratio shows retention in brain 4 days after administration of both liposomal formulations. The dotted line at a ratio of 1.4, indicates that the amount in brain equals the amount originating from blood <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0082331#pone.0082331-Dai1" target="_blank">[36]</a>. Mean concentrations ± SEM are plotted in all graphs. Statistical analysis using an unpaired two-tailed t-test showed that there was a significant difference in the plasma concentrations of 2B3-101 and PEG-lipo-DOX (p = 0.021) 4 days after administration, but not in the brain concentrations (p = 0.052), and not in the brain∶plasma ratios (p = 0.29).