Roflumilast N-oxide associated with PGE₂ partly inhibits chemokines release from A549 cells stimulated with CSE+LPS.

<p>Cells were preincubated with vehicle (blank bars) or 10 nM PGE₂ with roflumilast N-oxide at 1 nM (A, B and C) or 1 µM (D, E and F) (hatched bars) for 2 h and then stimulated or not with CSE at 2% or 4% in combination with LPS at 0.1 µg/ml. After 24 hours cell culture supernatants were collected and chemokines were quantified by ELISA. Results are expressed as means ± SEM of 3 independent experiments. * p<0.05 compared to control (0.01% DMSO); # p<0.05 compared to vehicle.