IL-6 production is higher in PBLs than in PBMCs.

<p>PBMCs and PBLs were prepared as described in the Materials and Methods, stimulated for 1, 3 or 6-12 (4 ng/mL), IL-15 (10 ng/mL), IL-18 (40 ng/mL), TL1A (100 ng/mL) and TL1AAb (1 µg/mL) and IL-6 was measured in the supernatants. (A) PBMCs and PBLs were stimulated with different combinations of cytokines and IL-6 was measured on day 6. (B) IL-6 production by PBMCs and PBLs stimulated with IL-12, IL-15, IL-18 and TL1A for 1, 3 and 6 days. (C) IL-6 production after 1, 3 and 6 days by PBLs and PBMCs stimulated with different cytokine combinations. Error bars represent the SEM of two measurements. Data are representative of three different experiments with cells from three separate donors.