IL-6 produced by PBLs depleted for CD4⁺, CD8⁺, HLA-DR⁺ or CD56⁺/CD16⁺ cells.

<p>(A) Freshly purified PBLs stained for CD4, CD8, CD3, HLA-DR, CD16, CD56. Depletion controls for PBLs depleted for CD4⁺, CD8⁺, HLA-DR⁺ or CD16⁺/CD56⁺ cells. PBLs and depletions were stimulated for 7 days with combinations of IL-12 (4 ng/mL), IL-15 (10 ng/mL), IL-18 (40 ng/mL) and TL1A (100 ng/mL) and stained for CD4, CD8, CD3, HLA-DR, CD16 and CD56 expression. (B) IL-6 production after 7 days by PBLs and depletions stimulated with IL-12, IL-15, IL-18 and TL1A as described above. Error bars represent the SEM of two measurements. Statistically significant differences by t-test: *** = p<0.001, * = p<0.05, n.s. = not significant. Data are representative of two different experiments with cells from two separate donors.