Leucine supplementation reduces ubiquitinated protein accumulation.

<p><b>A</b>: Representative Western blots of ubiquitinated proteins. Samples were analysed at two different time points of experimental protocol (3 and 10 days post-cryolesion). Muscle groups and experimental time points are identified at the top. <i>C</i>, intact control muscle; <i>Leu</i>, control muscles supplemented only with leucine from the time points of post-cryolesion day 3 and 10, respectively; <i>Cryo</i>, damaged muscle analysed on post-cryolesion days 3 and 10, respectively; and <i>Cryo+Leu</i>, leucine-supplemented damaged muscle analysed on post-cryolesion days 3 and 10, respectively. <b>B</b>: Densitometry analyses of ubiquitinated proteins at 3 (panel <i>a</i>) and 10 days (panel <i>b</i>) of experimental protocol. Blots were reacted with antibody specific for ubiquitin. Mw: molecular weight. Data are presented as mean±SD (n = 4). ^a<i>p</i><0.05 vs. C and Leu; ^b<i>p</i><0.05 vs. Cryo.