Effects of Rad21 or CTCF knockdown on expression of specific KSHV genes.

<p>CTCF or Rad21 knockdown was performed in iSLK cells with siRNAs specific for CTCF (CTCF Si) or Rad21 (Rad21 Si). Cells were also transfected with negative control siRNAs (NC Si). KSHV replication was induced with doxycycline (+D) or mock-induced (−D), and RNA was harvested 48 h after induction. qPCR was performed with primers specific for ORF57 (A), vIRF-1 (B), ORF47 (C), K2 (D) and K5 (E). Expression of each mRNA (RQ) was normalized to the level of expression in uninduced control cells (NC Si −D).