Cytokine determination in culture supernatants at different time frames.

<p>Cytokines were evaluated by magnetic bead-based multiplex immunoassays. The analysis was performed at day 4 and at day 4+4 on three series of culture supernatants. Data are expressed as mean concentration (ng/mL) ± SD. Only the histograms of the cytokines mainly involved in the effects evaluated in the study are reported in the figure. Grey bars: unstimulated cells; Black bars: PHA stimulated cells. Statistical significance was calculated using one-way analysis of variance (ANOVA) and Bonferroni post-test. *p<0.05.