<i>In vivo</i> homeostasis of A20-deficient mast cells.

<p>(A) Representative immunofluorescence images of whole-mount ear explants: green, avidin-FITC; red, anti-laminin; scale bar, 100 µm. Scatter plot shows proportions of mast cells (CD45⁺FcεRI⁺c-Kit⁺) in ear skin digests as identified by flow cytometric analysis. Bars indicate means from at least eight mice per genotype (Control, 7 <i>Mcpt5Cre</i>, 3 Cre⁻ littermates). (B) Dot plots showing proportions of ex vivo isolated peritoneal mast cells (FcεRI⁺c-Kit^hi). Numbers represent means ± SD from at least 20 mice per genotype (Control, 15 <i>Mcpt5Cre</i>, 5 Cre⁻ littermates). (C) Median fluorescent intensities (MFIs) of various cell surface markers expressed on A20-deficient and control <i>ex vivo</i> isolated peritoneal mast cells. Data are means + SD from at least four mice per genotype (Control, 4 <i>Mcpt5Cre</i>, 1 Cre⁻ littermate). (D) Scatter plots show absolute numbers of peritoneal mast cells (FcεRI⁺c-Kit^hi) and total peritoneal cells. Bars indicate means from at least 10 mice per genotype (Control, 8 <i>Mcpt5Cre</i>, 5 Cre⁻ littermates). (E) Scatter plots show proportions of cytokine positive peritoneal mast cells (c-Kit⁺) as identified by intracellular flow cytometric analysis. Bars indicate means from at least eight mice per genotype (Control, 9 <i>Mcpt5Cre</i>, 2 Cre⁻ littermates). *<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001 (one-way ANOVA).