Isolation and Characterisation of mouse MDSCs.

<p>(A) Phase-contrast micrographs of undifferentiated PP6 cells isolated from mouse skeletal muscle. The cells had a rounded shape like stem cells. (B) Growth curve of PP6 cells showed a slow-cycling phenotype like stem cells. (C) Immunofluorescence staining showed the PP6 cells were desmin and Sca-1 positive which indicated they were MDSCs. (D) Flow cytometry assay demonstrated that 93.23±0.93% of the PP6 cells were Sca 1 positive, and 94.18±0.38% were desmin positive. 90.1±1.28% were double positive cells (data are mean % cells±S.E.M.). All experiments were repeated at least three times in triplicates.