RNF146 overexpression in NSCLC.

<p>Detection of mRNA and protein expression of RNF146 in 20 NSCLC and control samples by RT-PCR (A) and Western blot (B). T: tumor samples; N: matched normal tissues; M: Marker. (C) mRNA and protein expression of RNF146 in the HBE cell line and the human NSCLC cell lines LK2, LH7 (squamous cancer), H1299, LTE, A549, and SPC (adenocarcinoma). GAPDH and β-actin served as internal controls. (D) Expression and distribution of RNF146 in NSCLC by immunohistochemistry. D1, RNF146 was not expressed in the normal bronchial and alveolar epithelium. D2, RNF146 was expressed in the cytoplasm of lung cancer cells, and weak RNF146 expression was detected in the adjacent bronchial epithelium. D3 and D5 showed weak RNF146 staining in the well-differentiated squamous cell carcinoma and adenocarcinoma. D4 and D6 showed strong RNF146 staining in the poorly differentiated squamous cell carcinoma and adenocarcinoma. (E) Relationship between RNF146 expression and the prognosis of NSCLC patients. E1 showed correlation between RNF146 expression and prognosis of stage I patients (<i>P</i><0.05). E2 and E3, no correlation between RNF146 expression and prognosis of stage II and stage III patients (<i>P></i>0.05).