Effects of HspBP1 and BAG-1M on MR transactivation.

<p>The impact of HspBP1 and BAG-1M on GR function was assessed by reporter gene experiments in Cos-7 cells. Control and reporter plasmids were transfected together with plasmids expressing MR and wild-type or mutant HspBP1 or BAG-1M at the indicated amounts. 24 h after transfection, cells were treated with hormones at the indicated concentrations. In the left panel, the vector control (i.e. “empty” expression vector) was set to 100% (dashed line). In the right panel, vector control at the highest concentration of hormone was arbitrarily set to 100%. Error bars indicate the standard error of the mean (+SEM) of four independent experiments performed in triplicates (A). Symbols indicate significant differences to vector control for BAG-1M (+), BAG1M_mut ($), HspBP1 (*), and HspBP1_mut (#), with p<0.05, 0.01, 0.001 for one, two or three symbols, respectively. Representative Western blots are shown in B and C for the indicated conditions.